Biotin, Biotin Solution, BD Solution, Biotin Decoy Solution
Cell cultures aimed at obtaining specific ligands such as monoclonal antibodies are treated with biotin as a nutrient additive. Today, obtaining those ligands with maximum purity involves a long and ineffective method: The biotin found in the cell culture naturally has a very high affinity for the avidin in the chromatography column; If the biotins are not removed from the culture before it passes through the column, they will bind to the avidin and break off the biotinylated receptors. Because of this, fewer active and stable receptors are available to capture the ligands of interest. As a result, precious ligands are lost and a larger chromatography column is necessary to obtain adequate amounts.
